Midv-075

RNA extracts from pooled mosquito samples underwent unbiased metagenomic sequencing on an Illumina NovaSeq platform. Bioinformatic pipelines (Kraken2, Diamond, and custom BLAST‑n searches) flagged a contig of ~11 kb that bore only distant similarity (≈42 % nucleotide identity) to known Toscana and Bunyamwera viruses. Subsequent Sanger validation confirmed the presence of a distinct viral genome, which the team provisionally named Midge‑borne Insect‑derived Virus (MIDV). The isolate from sample 075—originating from a night‑time collection near the Vam Nao River—received the accession number .

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