Instead of manual trial-and-error, you input compound properties (Log D, pKa), and the software predicts retention times. It then automatically writes the optimized HPLC program for your system.

Detail the %B changes over time (e.g., 0 min: 30%, 15 min: 70%). Flow Rate: Usually expressed in mL/min (e.g., 1.0 mL/min).

: To provide a highly purified sample for the quantification of multiple steroids (such as E2, E1, P4, and DHEA) from very small volumes of biofluid.