A straight line is not always right. If your data shows heteroscedasticity (common in HPLC-UV or LC-MS/MS), M22-A3 instructs you to use weighted regression. Using unweighted 1/x will cause bias in the lower range.
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The CLSI M22-A3 document covers various aspects of blood culture collection, including: A straight line is not always right
The CLSI M22-A3 guideline covers essential topics, including: labs frequently make errors in implementation.
Even if you have the CLSI M22-A3 PDF on your desk, labs frequently make errors in implementation.